| 专利名称 | Method for increasing the efficiency of inducing pluripotent stem cells | 申请号 | US201213808596 | 专利类型 | US | 公开（公告）号 | US9163217(B2) | 公开（授权）日 | 2015.10.20 | 申请（专利权）人 | 中国科学院广州生物医药与健康研究院 | 发明（设计）人 | Pei Duanqing;Wang Tao | 主分类号 | C12N15/00 | IPC主分类号 | C12N15/00;C12N5/0775;C12N5/074 | 专利有效期 | Method for increasing the efficiency of inducing pluripotent stem cells 至Method for increasing the efficiency of inducing pluripotent stem cells | 法律状态 | 说明书摘要 | The present invention relates to a method for increasing the efficiency of inducing pluripotent stem cells by utilizing genes Jhdm1b and Jhdm1a that modify histone. By utilizing Jhdm1b, Jhdm1a, and a stem cell inducing factor, the present invention increases the efficiency of inducing pluripotent stem cells and increases the quality of induced pluripotent stem cells. The stem cell inducing factor is a combination of Oct4 and Klf4, or a combination of Sox2, Oct4, and Klf4, or a combination of Oct4 and Sox2, and Oct4 alone. The method further comprises exposing the cells to vitamin C, which further increases the efficiency of inducing pluripotent stem cells as compared with the case where no vitamin C is used. By using less stem cell reducing factors, the method of the present invention reduces the potential carcinogenicity, obtains a high inducing efficiency, and provides high-quality induced pluripotent stem cells capable of germ-line transmission. |
1、源头对接，价格透明
2、平台验证，实名审核
3、合同监控，代办手续
4、专员跟进，交易保障