METHOD AND SYSTEM FOR SCREENING THE RECEPTOR-LIGAND BINDING IN LIVE CELL

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专利名称 METHOD AND SYSTEM FOR SCREENING THE RECEPTOR-LIGAND BINDING IN LIVE CELL 申请号 EP20070721024 专利类型 EP 公开(公告)号 EP2023138(B1) 公开(授权)日 2012.03.21 申请(专利权)人 中国科学院上海生命科学研究院 发明(设计)人 ZHOU ZHUANCHENG HEPIN 主分类号 G01N33/50 IPC主分类号 G01N33/50 专利有效期 METHOD AND SYSTEM FOR SCREENING THE RECEPTOR-LIGAND BINDING IN LIVE CELL 至METHOD AND SYSTEM FOR SCREENING THE RECEPTOR-LIGAND BINDING IN LIVE CELL 法律状态 说明书摘要 The invention relates to methods and system for detecting ligand binding to membrane receptors and endocytosis. A method for detecting ligand binding to a membrane receptor includes the steps of: incubating the cell with a dye and a ligand; and detecting dye-containing endocytic vesicles in the cell. A system for screening ligand binding to membrane receptors includes an automatic liquid handling device; an x-y stage for positioning a plate; a microscope configured to image endocytic vesicles of a cell; a software for automatic analysis of FM spots (endocytic vesicles); and a control unit configured to control the movement of the x-y stage and the microscope.

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