专利名称 | METHOD AND SYSTEM FOR SCREENING THE RECEPTOR-LIGAND BINDING IN LIVE CELL | 申请号 | EP20070721024 | 专利类型 | EP | 公开(公告)号 | EP2023138(B1) | 公开(授权)日 | 2012.03.21 | 申请(专利权)人 | 中国科学院上海生命科学研究院 | 发明(设计)人 | ZHOU ZHUANCHENG HEPIN | 主分类号 | G01N33/50 | IPC主分类号 | G01N33/50 | 专利有效期 | METHOD AND SYSTEM FOR SCREENING THE RECEPTOR-LIGAND BINDING IN LIVE CELL 至METHOD AND SYSTEM FOR SCREENING THE RECEPTOR-LIGAND BINDING IN LIVE CELL | 法律状态 | 说明书摘要 | The invention relates to methods and system for detecting ligand binding to membrane receptors and endocytosis. A method for detecting ligand binding to a membrane receptor includes the steps of: incubating the cell with a dye and a ligand; and detecting dye-containing endocytic vesicles in the cell. A system for screening ligand binding to membrane receptors includes an automatic liquid handling device; an x-y stage for positioning a plate; a microscope configured to image endocytic vesicles of a cell; a software for automatic analysis of FM spots (endocytic vesicles); and a control unit configured to control the movement of the x-y stage and the microscope. |
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