| 专利名称 | Method for detecting negatively supercoiled DNA | 申请号 | US20030699852 | 专利类型 | US | 公开（公告）号 | US2004235007 | 公开（授权）日 | 2004.11.25 | 申请（专利权）人 | 中国科学院遗传与发育生物学研究所 | 发明（设计）人 | HIROSE SUSUMU (JP); MATSUMOTO KUNIHARU (JP) | 主分类号 | C12Q1/68 | IPC主分类号 | C12Q1/68;G01N33/53 | 专利有效期 | Method for detecting negatively supercoiled DNA 至Method for detecting negatively supercoiled DNA | 法律状态 | 说明书摘要 | To detect intracellular negatively supercoiled DNA conveniently and efficiently. A method for detecting negatively supercoiled DNA in cells, characterized by including the steps of incorporating biotinylated psoralen into cells, irradiating the cells with long-wavelength UV rays, causing the cells to react with adivin which has been labeled with a color-developing substance, a fluorescent substance, or a chemiluminescent substance, and measuring developed color, emitted fluorescence, or emitted chemiluminescence of the cells. |
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