| 专利名称 | Bacillus stearothermophilus DNA polymerase with proof-reading 3'-5' exonuclease activity | 申请号 | US64268496A | 专利类型 | US | 公开（公告）号 | US5834253A | 公开（授权）日 | 1998.11.10 | 申请（专利权）人 | 中国科学院上海生物化学研究所 | 发明（设计）人 | HONG GUO FAN ; HUANG WEI-HUA ; ZHAI DECEASED FENG | 主分类号 | C12N9/12 | IPC主分类号 | C12N9/12;C12N15/54;C12P19/34 | 专利有效期 | Bacillus stearothermophilus DNA polymerase with proof-reading 3'-5' exonuclease activity 至Bacillus stearothermophilus DNA polymerase with proof-reading 3'-5' exonuclease activity | 法律状态 | 说明书摘要 | The invention relates to DNA polymerases which are capable of proofreading 3'-5' exonuclease activity during DNA sequencing of a DNA strand, such that the DNA polymerase functions to excise mismatched nucleotides from the 3' terminus of the DNA strand at a faster rate than the rate at which the DNA polymerase functions to remove nucleotides matched correctly with the nucleotides of the template, and which DNA polymerase does not exhibit 5'-3' exonuclease activity. The invention also relates to isolated and cloned DNA sequences derived from the Bacillus stearothermophilus thermostable DNA polymerase, as well as the expressed polymerase itself. |
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